Premium
Fluorescence labeling of mouse alloantibodies
Author(s) -
Hämmerling U.,
Chin A. F.,
Arny M.
Publication year - 1974
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830040416
Subject(s) - caproic acid , fluorescence , fluorescein , biology , amide , antibody , biochemistry , microbiology and biotechnology , chemistry , immunology , physics , quantum mechanics , food science
Abstract Mouse alloantibodies can be readily labeled with fluoresceinyl thioureido caproic acid and tetramethylrhodaminylthiourido caproic acid, which are new derivatives of fluorescein or tetramethylrhodamine, containing a caproic acid residue as a spacer. The ω‐carboxyl group will establish an amide bond with amino groups of portein in a mild condensation reaction with water‐soluble carbodiimides. Antibody activity is fully retained, and binding to fixed or unfixed cells yields specific fluorescence of a practical intensity.