Premium
Hapten‐induced paralysis. I. Evidence for the persistence of hapten‐specific immunocompetent B lymphocytes in specifically paralyzed mice
Author(s) -
Gronowicz Eva,
Coutinho A.,
Sjöberg O.
Publication year - 1974
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830040314
Subject(s) - hapten , in vivo , in vitro , paralysis , immunology , biology , spleen , antibody , medicine , biochemistry , surgery , microbiology and biotechnology
Abstract Injection of 3,5‐dinitro‐4‐hydroxy‐phenacetyl (NNP) azide induced specific unresponsiveness, as judged by the failure of pretreated mice to respond to NNP‐HRC (horse red blood cells), but not to HRC. The unresponsiveness of the NNP azide‐treated mice was not broken by incubating lymphocytes in vitro together with NNP‐HRC. Injection of the nonreactive hapten, NNP‐ϵ‐aminocaproic acid, also induced specific unresponsiveness when measured in vivo , but cells from such animals did respond normally in vitro . Spleen cells from mice treated with NNP azide contained a higher number of background PFC to NNP and NIP as compared to untreated controls.