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The susceptibility of the mouse immunoglobulin subclasses to suppression by high doses of antigen
Author(s) -
Hay F. C.,
Torrigiani G.
Publication year - 1974
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830040103
Subject(s) - subclass , bordetella pertussis , biology , antibody , antigen , antiserum , immunology , immunoglobulin g , genetics , bacteria
Abstract Using the coprecipitation technique and rabbit antisera specific for mouse IgG1, IgG2a, IgG2b, IgA and IgM, the responses of BALB/c mice to a wide range of doses of human serum albumin have been studied. Antibody activity was detected in all five classes. At lower doses, below 1 mg, the distribution of antibody activity among the immunoglobulin classes was fairly constant, but at higher doses a decrease in antibody activity occurred in certain subclasses only, particularly IgG1. The actual effect obtained depended on whether the antigen was given with or without Bordetella pertussis . To study the subclass suppression further, mice were also subjected to neonatal treatment with soluble antigen. The suppression here was again mainly in the thymus‐dependent IgG1 subclass.