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Stimulation of DNA synthesis in mouse lymphoid cells by polyanions in vitro. II. Relationship between adjuvant activity and stimulation of DNA synthesis by polyanions
Author(s) -
Vogt W.,
Rühl H.,
Wagner B.,
Diamantstein T.
Publication year - 1973
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830030808
Subject(s) - dna synthesis , in vivo , in vitro , stimulation , biology , dextran , spleen , dna , adjuvant , microbiology and biotechnology , biochemistry , immunology , endocrinology , genetics
Abstract The effect of eleven different polyanions, among them dextran sulfate, and of two different preparations of dextran was investigated on [ 3 H]dThd incorporation in spleen cells in vitro , and on PFC response to SRBC in vivo. In general, polyanions stimulated DNA synthesis in spleen cells and enhanced PFC response to SRBC. Dextran itself had no effect on DNA synthesis and on PFC response. The degree of activation of DNA synthesis by polyanions in vitro was found to correlate with the adjuvant activity of the substances in vivo It is postulated that activation of DNA synthesis in lymphoid cells is essential for the adjuvant activity of polyanions.