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Genetic control of phytohemagglutinin responsiveness by murine spleen cells and correlation between the release of lymphotoxins and dna synthesis
Author(s) -
Donner Mireille,
Vaillier D.,
Burg C.
Publication year - 1973
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830030710
Subject(s) - congenic , spleen , biology , dna , dna synthesis , microbiology and biotechnology , stimulation , locus (genetics) , negative control , immunology , genetics , gene , endocrinology , medicine , traditional medicine
Abstract Response to phytohemagglutinin was investigated in adult mouse spleen cells of congenic strains which differ only at the H‐2 locus. The stimulation of spleen cells was assessed by release of lymphotoxins and DNA synthesis. The results obtained show that the response to PHA markedly varied according to the genetic origin of spleen cells. Cells with H‐2 a and H‐2 b specificities responded better to PHA than those with H‐2 f and H‐2 s specificities. Furthermore, there was good correlation between the amount of released lymphotoxins and the level of DNA synthetic response. These results are discussed on the basis of an H‐2 linked genetic control of PHA response in mouse strains.