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Affinity and Ig classes of anti‐hapten antibodies in carrier‐preimmunized rats
Author(s) -
Hurme M.,
Kontiainen Sirkka,
Seppälä I. J. T.,
Mäkelä O.
Publication year - 1973
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830030403
Subject(s) - hapten , conjugate , titer , priming (agriculture) , biology , antibody , immunology , antibody titer , microbiology and biotechnology , mathematical analysis , botany , germination , mathematics
Abstract Priming rats with the carrier protein 7 – 43 days before injecting them with a conjugate of hapten NIP [(4‐hydroxy‐5‐iodo‐3‐nitrophenyl)acetyl] and the protein accelerated the anti‐hapten response: peak titers were reached sooner, the replacement of IgM by IgG antibody happened earlier and the affinity of anti‐NIP was higher in carrier‐primed than in non‐carrier‐primed rats. The acceleration effect on peak titers was short‐lasting. By day 9 the anti‐hapten titers in non‐carrier‐primed animals had reached those of the carrier‐primed animals. The difference in affinities gradually decreased, but was still demonstrable 28 days after immunization with the conjugate.