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Migration inhibition experiments with mixtures of human peripheral blood lymphocytes and guinea pig peritoneal exudate cells
Author(s) -
Marsman A. J. W.,
Van Der Hart Mia,
Walig C.,
Eijsvoogel V. P.
Publication year - 1972
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830020614
Subject(s) - in vitro , biology , guinea pig , tuberculin , delayed hypersensitivity , immunology , immune system , antigen , peripheral blood , candida albicans , lymphocyte , exudate , microbiology and biotechnology , pathology , medicine , endocrinology , biochemistry , tuberculosis , botany
Abstract Migration inhibition using mixtures of human peripheral blood lymphocytes and non‐sensitized guinea pig peritoneal exudate cells was demonstrable with purified protein derivative of tuberculin (PPD) and Candida albicans extract (CAE) when lymphocytes were obtained from individuals with a delayed type skin reaction to these antigens. No migration inhibition occurred when lymphocytes were used from skin test negative individuals. Furthermore it was found that lymphocytes from some skin test negative persons, which did not induce migration inhibition, showed, however, an increased DNA synthesis when cultured in vitro with PPD. This indicates that a dissociation between the results of migration inhibition, skin test and lymphocyte transformation can occur not only in patients with immune disorders, as has been described by other investigators, but also in healthy individuals.