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Sulfanilic acid as surface antigen on mouse lymphoid cells. I. Properties of hapten‐labeled cells and effects of antihapten antiserum in vitro and in vivo
Author(s) -
Seiler H.
Publication year - 1972
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830020612
Subject(s) - hapten , in vivo , biology , antiserum , antigen , antibody , in vitro , sulfanilic acid , microbiology and biotechnology , immunology , biochemistry , ecology
Abstract The conventional hapten sulfanilic acid (sulf) was coupled covalently to mouse spleen cells via an azobridge. Survival and functional behavior of the coupled cells were assayed by determining their capacity to exclude trypan blue, to secrete antibody and to produce a secondary immune response in an adoptive transfer system. Conditions have been found under which the cells were not affected by the coupling procedure but could be specifically lysed or inactivated in vitro and in vivo by anti‐sulf antiserum. Inactivation in vivo appeared to require complement, since F(ab') 2 fragments of anti‐sulf antibody did not inactivate coupled cells. The effects of antibody and complement were shown to depend strongly on the amount of hapten coupled to the cells. The potential of this method is twofold. The hapten can serve as a surface marker, which can be detected by the effect of antiserum in vitro and in vivo . In addition the coupled hapten may be used as an artificial histocompatibility antigen, which can be manipulated to a certain extent for experimental analysis of its function.