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Protease activity as a possible mechanism of migration inhibitory factor
Author(s) -
Havemann K.,
Howat M.,
Sodomann C. P.,
Havemann Karin,
Gürger Sigrid
Publication year - 1972
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830020118
Subject(s) - proteases , diisopropyl fluorophosphate , protease , biology , inhibitory postsynaptic potential , macrophage migration inhibitory factor , protease inhibitor (pharmacology) , venom , biochemistry , microbiology and biotechnology , enzyme , immunology , human immunodeficiency virus (hiv) , neuroscience , cytokine , antiretroviral therapy , viral load
Abstract The activity of migration inhibitory factor (MIF), present in the supernatants of specifically and nonspecifically stimulated human lymphocyte cultures, can be blocked by antiproteases of animal and plant origin and by diisopropyl fluorophosphate. MIF, partially purified by column chromatography and zone electrophoresis on polyvinylchloride is blocked in the same way. On the other hand, migration inhibition can be effected by some proteases, especially by cobra venom. These findings are discussed in terms of the probable protease nature of MIF.