Premium
Immunological purification and chemical characterization of the group‐specific proteins (Gc) from human serum
Author(s) -
Ruoslahti E.,
Simons K.,
Ehnholm C.
Publication year - 1971
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830010109
Subject(s) - biology , peptide , biochemistry , microbiology and biotechnology , gene , locus (genetics) , chromatography , chemistry
Abstract The group‐specific (Gc) proteins have been purified by an immunochemical method from human sera of Gc 1–1 and Gc 2–2 phenotypes. The two electrophoretic components of the Gc 1 protein, Gc 1F and Gc 1S were isolated by electro‐focusing. Tryptic peptide maps on cellulose thin layers of Gc 1, Gc 1F, Gc 1S and Gc 2 revealed one peptide specific for Gc 2, another peptide specific for Gc 1 and a third characteristic for Gc 1F. The amino acid composition of these peptides was determined. From these and previous results a hypothesis based on one gene locus is put forward to explain the Gc polymorphism.