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Effects of middle‐level static magnetic field on metabolic activity of mitochondria
Author(s) -
Yamashita Kazuhiko,
Saito Masao
Publication year - 2001
Publication title -
electrical engineering in japan
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.136
H-Index - 28
eISSN - 1520-6416
pISSN - 0424-7760
DOI - 10.1002/eej.1078
Subject(s) - mitochondrion , respiration , cell , magnetic field , energy metabolism , cell cycle , microbiology and biotechnology , biology , chemistry , biophysics , physics , biochemistry , endocrinology , anatomy , quantum mechanics
This study intends to clarify the effect of a static magnetic field on cellular activities. It is concerned with the metabolic activity of mitochondria from the viewpoint of their energy level. As a preliminary examination, the time required to render the cells to the low‐energy state (G0/G1 phase of the cell cycle) and to reduce their energy level was examined by maintaining the cells in medium with low‐density fetal bovine serum. Then, we examined the effect of the magnetic field on mitochondrial energy activity from the viewpoint of cell metabolism estimated by the MTT assay. We also examined the cell respiration, expecting that the mitochondrial energy activity level would be reflected in the respiration. The magnetic field was kept at 100 mT, with 0 mT as the control. The results of the MTT assay indicated that the mitochondrial energy activity was enhanced by a factor of 1.5. We found that the cell respiration was increased by a factor of 1.3 on exposure to the magnetic field. The t‐test showed the difference to be statistically significant. It is thus concluded that mitochondrial energy activity is enhanced in the G0/G1 phase of the cell cycle by exposure to a magnetic field. On the other hand, we also found that the mitochondrial energy cycle period is little affected. © 2001 Scripta Technica, Electr Eng Jpn, 137(1): 36–41, 2001