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Photo caption and credit Caption (Upper Left): Fly agaric (Amanita muscaria) in a restored forest of the Shiretoko National Park, JapanPhoto credit: Shinichi Tatsumi. Caption (Upper Middle): Example cotton strip setup with temperature logger, following 7‐10 days in the water, as deployed in the Waikato Region, New Zealand to study the ecological health of non‐wadeable rivers (see Wood et al., this issue) Photo credit: Mark Hamer & Michael Pingram, Waikato Regional Council Caption (Upper Right): Lesperance et al. report a statistically robust model for calibration and computation of detection and quantification limits for low copy number environmental DNA samples. Photo credit: Caden Churchill and Caren Helbing Caption (Lower Left): Fish communities on the St. Charles River, Québec, based on eDNA metabarcoding analyses Photo credit: Louis Bernatchez Caption (Lower Middle): A giant otter from the Kaw‐Roura Marshes Natural National Reserve in French Guiana Photo credit: Marie Aucourd Caption (Lower Right): First barcode of the Heterocypris salina, living shallow water ostracod female (♀), from Lake Nam Co, Tibetan Plateau. (left) Light miscroscopy picture of the left valve, internal view and soft parts. (right) scanning electron microscopy (SEM) picture of the right carapace in external view. Photo credit: Paula Echeverría‐Galindo, Mauricio Bonilla and Peter Frenzel