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Photo caption and credit Caption (Upper Left): Caption: Domestic cattle in the Mahadeshwara Hills Wildlife Sanctuary in southern India Photo credit: Rajindra Puri Caption (Upper Middle): Sampling site for invasive brown trout (Salmo trutta) and native galaxiids (Aplochiton spp.) eDNA at Findley Creek (Falkland Islands, 2018) Photo credit: F. Minett Caption (Upper Right): A bumble bee (Bombus sp.) with visible pollen load visiting Salvia sp. (Lamiaceae) flowers. Photo credit: Madison Marshal Caption: (Lower Left): Frog species (Euparkerella brasiliensis) that can be found in the leaf litter of Reserva BiolÓgica de GuapiaÇu, Brazil Photo credit: Tiago Silva‐Soares Caption (Lower Middle): eDNA detection of metazoans in sedimented tropical reef environments Photo credit: Huang Danwei Caption (Lower Right): Langlois et al. discuss the need for robust qPCR‐based eDNA detection assays in environmental monitoring and species inventories. Photo credit: Michael Allison