Open AccessRemoval of melatonin receptor type 1 signalling induces dyslipidaemia and hormonal changes in mice subjected to environmental circadian disruption
Author(s) -
Tchio Cynthia,
Baba Kenkichi,
Piccione Giuseppe,
Tosini Gianluca
Publication year - 2021
Publication title -
endocrinology, diabetes and metabolism
Language(s) - English
Resource type - Journals
ISSN - 2398-9238
DOI - 10.1002/edm2.171
Subject(s) - medicine , endocrinology , melatonin , ghrelin , leptin , hormone , circadian rhythm , resistin , biology , receptor , glucose homeostasis , lipid metabolism , insulin , adiponectin , insulin resistance , obesity
Background Melatonin is a hormone secreted by the pineal gland in a circadian rhythmic manner with peak synthesis at night. Melatonin signalling was suggested to play a critical role in metabolism during the circadian disruption. Methods Melatonin‐proficient (C3H‐f +/+ or WT) and melatonin receptor type 1 knockout (MT 1 KO) male and female mice were phase‐advanced (6 hours) once a week for 6 weeks. Every week, we measured weight, food intake and basal glucose levels. At the end of the experiment, we sacrificed the animals and measured the blood's plasma for lipids profile (total lipids, phospholipids, triglycerides and total cholesterol), metabolic hormones profiles (ghrelin, leptin, insulin, glucagon, glucagon‐like‐peptide and resistin) and the body composition. Results Environmental circadian disruption (ECD) did not produce any significant effects in C3H‐f +/+ , while it increased lipids profile in MT 1 KO with the significant increase observed in total lipids and triglycerides. For metabolic hormones profile, ECD decreased plasma ghrelin and increased plasma insulin in MT 1 KO females. Under control condition, MT 1 KO females have significantly different body weight, fat mass, total lipids and total cholesterol than the control C3H‐f +/+ females. Conclusion Our data show that melatonin‐proficient mice are not affected by ECD. When the MT 1 receptors are removed, ECD induced dyslipidaemia in males and females with females experiencing the most adverse effect. Overall, our data demonstrate that MT 1 signalling is an essential modulator of lipid and metabolic homeostasis during ECD.