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Natural abundance of 13 C and 15 N provides evidence for plant–soil carbon and nitrogen dynamics in a N‐fertilized meadow
Author(s) -
Wang Ruzhen,
Peñuelas Josep,
Li Tian,
Liu Heyong,
Wu Hui,
Zhang Yuge,
Sardans Jordi,
Jiang Yong
Publication year - 2021
Publication title -
ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.144
H-Index - 294
eISSN - 1939-9170
pISSN - 0012-9658
DOI - 10.1002/ecy.3348
Subject(s) - mineralization (soil science) , nitrification , nitrogen , nitrogen cycle , chemistry , ammonium , soil carbon , cycling , isotopes of nitrogen , ecosystem , environmental chemistry , biomass (ecology) , agronomy , ecology , soil water , biology , forestry , organic chemistry , geography
Natural abundance of carbon (C) and nitrogen (N) stable isotope ratios (δ 13 C and δ 15 N) has been used to indicate ecosystem C and N status and cycling; however, use of this approach to infer plant and microbial N preference under projected ecosystem N enrichment is limited. Here, we investigated natural abundance δ 13 C and δ 15 N of five dominant plant species, and soil δ 15 N of microbial biomass and available N forms under N addition in a meadow steppe. Additional N, applied as urea, led to decreases in δ 15 N of soil NO 3 − (δ 15 N nitrate , from 3.0 to 0.4‰) and increases in δ 15 N of soil NH 4 + (δ 15 N ammonium , from −1.3 to 11‰) and dissolved organic N (δ 15 N DON , from 8.5 to 15‰) that reflected increased net nitrification rates, a possible increase in NH 3 volatilization, and greater availability of the three N forms. An overall increase in δ 15 N of soil total N (δ 15 N TN ) from 7.1 to 7.9‰ indicated accelerated and greater openness of soil N cycling that was also partially revealed by enhanced net N mineralization rates. Plant δ 15 N, which ranged from −1.8 to 2.1‰, generally decreased with N addition, indicating a greater reliance on soil NO 3 − under N‐enrichment conditions. Nitrogen addition decreased δ 15 N of microbial biomass N (from 14 to 2.8‰), possibly because of a shift in preferential N form (DON to NO 3 ‐ ), that indicated a convergence of plant and microbial preferential N forms and an increase in plant–microbial N competition. Microbes were thus more flexible than plants in the use of different forms of N. Addition of N decreased plant litter δ 13 C, whereas plant species δ 13 C remained unaffected, likely because of a shift in the abundance of dominant species with a greater proportion of biomass coming from δ 13 C‐depleted species. Enrichment factor (the difference in plant δ 15 N relative to δ 15 N TN ) of four nonlegume species was negatively related to soil inorganic N availability, net nitrification rate, and net N mineralization rate, and was proven to be a good indicator of ecosystem N status. Our study highlights the importance of natural abundance of 15 N as an indicator of plant–microbial N competition and ecosystem N cycling in meadow steppe grasslands under projected ecosystem N enrichment.