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Development of novel ECM which has stiffness difference based on hydrogel beads
Author(s) -
Miyata Hiromu,
Peng Zugui,
Shimba Kenta,
Miyamoto Yoshitaka,
Yagi Tohru
Publication year - 2021
Publication title -
electronics and communications in japan
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.131
H-Index - 13
eISSN - 1942-9541
pISSN - 1942-9533
DOI - 10.1002/ecj.12321
Subject(s) - extracellular matrix , elasticity (physics) , tissue engineering , materials science , stiffness , elastic modulus , atomic force microscopy , c2c12 , composite material , young's modulus , self healing hydrogels , nanotechnology , biomedical engineering , chemistry , polymer chemistry , myocyte , engineering , microbiology and biotechnology , biochemistry , myogenesis , biology
Abstract For tissue engineering, it is important to understand the relationship between cells’ behavior and the mechanical properties of extracellular matrix (ECM). We aim to develop a novel ECM taking the advantage of micro‐sized polyacrylamide gel beads. The advantages of our ECM are creation of any elasticity at any position. Elasticity measurement experimental results by atomic force microscope (AFM) show that there is a significant difference in Young modulus between the place where gel beads exist and the place where gel beads do not exist. In addition, C2C12 cell culture experiments show that cell behavior changes with and without gel beads.