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Stimulations inducing tethered lipid bilayer formation on protein‐modified substrate
Author(s) -
Tero Ryugo,
Sato Junichi
Publication year - 2020
Publication title -
electronics and communications in japan
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.131
H-Index - 13
eISSN - 1942-9541
pISSN - 1942-9533
DOI - 10.1002/ecj.12262
Subject(s) - lipid bilayer , vesicle , lipid vesicle , bilayer , biophysics , substrate (aquarium) , biological membrane , membrane , streptavidin , chemistry , model lipid bilayer , lipid bilayer phase behavior , nanotechnology , materials science , biochemistry , biotin , biology , ecology
Supported lipid bilayer (SLB) is one artificial biomembrane system used to study transportation reactions through biomembranes using solid devices and surface scientific methods. A key step in the SLB formation is the transformation of spherical lipid vesicles to a planar membrane. We investigated stimulation methods for adsorbed lipid vesicles on a streptavidin‐modified SiO 2 /Si substrate to induce tethered‐type SLB. We describe the effects of osmotic stress, Ca 2+ addition, and a freeze–thaw cycle on the adsorbed vesicles, and on the condition of t‐SLB.