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Proposal of micro plasma extraction device by autonomous trigger control
Author(s) -
Naito Hiroki,
Okamoto Shunya,
Ukita Yoshiaki
Publication year - 2020
Publication title -
electronics and communications in japan
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.131
H-Index - 13
eISSN - 1942-9541
pISSN - 1942-9533
DOI - 10.1002/ecj.12261
Subject(s) - polydimethylsiloxane , microfluidics , fluidics , extraction (chemistry) , plasma , whole blood , biomedical engineering , materials science , chromatography , computer science , chemistry , nanotechnology , engineering , electrical engineering , physics , surgery , medicine , quantum mechanics
In this paper, we report on the development of a microfluidic device, which extracts plasma from low‐volume whole blood (10 μL), and is aimed at integration with microfluidic system for micro analytical systems. The device autonomously separates blood cells and transfers the separated plasma by steady rotation. Based on the original autonomous fluidic control theory, we developed the principle of automatic timed injection of pressurizing medium into the separation chamber. This realizes the transferring of separated plasma from blood separation chamber to the other place, after separating blood cells by centrifugal force. Through the demonstration on a polydimethylsiloxane microfluidic device, we confirmed that the automatic plasma extraction was successfully implemented as designed. The efficiency of separation was evaluated on the basis of the concentrations of blood cells in samples before and after extraction, and the rate of blood cell removal was 98.5%.