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Novel microfluidic device integrated with a fluidic‐capacitor to mimic heart beating for generation of functional liver organoids
Author(s) -
Wu Jiaxu,
Hirai Yoshikazu,
Kamei KenIchiro,
Tsuchiya Toshiyuki,
Tabata Osamu
Publication year - 2019
Publication title -
electronics and communications in japan
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.131
H-Index - 13
eISSN - 1942-9541
pISSN - 1942-9533
DOI - 10.1002/ecj.12210
Subject(s) - organoid , microfluidics , controllability , hydrostatic pressure , fluidics , computer science , nanotechnology , biomedical engineering , neuroscience , materials science , biology , engineering , electrical engineering , physics , thermodynamics , mathematics
Liver organoids hold a great potential to understand liver development and contribute for drug screening and toxicological testing. However, conventional methods to generate organoids provide insufficient liver functions and less reproducibility, due to lack of controllability of cellular microenvironmental cues. To tackle these issues, we focus on one of the environmental cues, pressure stimuli by heart beating, and develop a microfluidic‐based cell culture platform integrating a fluidic capacitor to produce pressure stimuli with hydrostatic pressure. Furthermore, we demonstrate a numerical simulation based on equivalent circuit model for designing the device parameters with sufficient accuracy. This device concept will provide insights into physical micro‐environmental regulation on organoid development.