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Development of micro‐inclined well array for trapping single cells
Author(s) -
Tsukamoto Takuya,
Furuya Naoki,
Shimagami Takuya,
Sato Rui,
Shimokawa Fusao,
Akimitsu Kazuya,
Suzuki Takaaki
Publication year - 2018
Publication title -
electronics and communications in japan
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.131
H-Index - 13
eISSN - 1942-9541
pISSN - 1942-9533
DOI - 10.1002/ecj.12117
Subject(s) - trapping , materials science , photolithography , trap (plumbing) , optics , optoelectronics , biology , engineering , ecology , physics , environmental engineering
We investigated the shape of a well to trap single cells for the purpose of improving the cell trapping rate after cell seeding and liquid exchange in a cell microarray. The wells having vertical and inclined tapered side‐wall were made using three‐dimensional UV photolithography. Changes in cell trapping rate by the shape of the wells were evaluated by trapping tests with fluorescent microbeads and living cells. As a result of trapping test with living cells, bead residual rate of the microarray with inclined tapered wells is up to 2.9 times higher than that with vertical wells.