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A Study for Sensitivity Improvement of 3‐D Lab‐on‐a‐CD‐Based Immunosensor
Author(s) -
KATAOKA CHIWA,
AZETA TSUKASA,
SAWADAISHI KAZUYUKI,
UKITA YOSHIAKI,
YAMAGUCHI AKINOBU,
UTSUMI YUICHI
Publication year - 2018
Publication title -
electronics and communications in japan
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.131
H-Index - 13
eISSN - 1942-9541
pISSN - 1942-9533
DOI - 10.1002/ecj.12023
Subject(s) - streptavidin , immunoassay , substrate (aquarium) , detection limit , biomolecule , chemistry , chromatography , biotin , antibody , biochemistry , biology , ecology , immunology
SUMMARY We have developed a three‐dimensional (3D) Lab‐on‐a‐CD immunosensor based on a competitive/noncompetitive assay for small endocrine disruptors and biomolecules. To improve the sensitivity of the 3D Lab‐on‐a‐CD immunosensor, an immobilizing method of capturing‐type antibody on the sensor surface was investigated. The method is based on the introduction of a highly reactive amino group to a P(MMA/CEMA) copolymer surface having chlorine, and utilizing a biotin‐streptavidin system. The functionality of the 3D Lab‐on‐a‐CD immunosensor was evaluated by measuring mouse IgG. The detection limit was found to be 3 f g/assay (3 × 10 ‐12 g/assay). After further modification, the 3D Lab‐on‐a‐CD immunosensor, having some characteristics such as a substrate with highly integrated antibodies, is expected to be applicable for highly sensitive immunoassay.