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Estimation of metabolic characteristics of heat‐injured bacteria by dielectrophoretic impedance measurement
Author(s) -
Amako Eri,
Enjoji Takaharu,
Uchida Satoshi,
Tochikubo Fumiyoshi
Publication year - 2011
Publication title -
electronics and communications in japan
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.131
H-Index - 13
eISSN - 1942-9541
pISSN - 1942-9533
DOI - 10.1002/ecj.10321
Subject(s) - biological system , conductance , electrical impedance , work (physics) , materials science , fermentation , escherichia coli , biochemical engineering , chemistry , computer science , thermodynamics , biochemistry , physics , biology , electrical engineering , engineering , condensed matter physics , gene
Constant monitoring and immediate control of fermentation processes have been required for advanced quality preservation in the food industry. In the present work, simple estimation of metabolic states for heat‐injured Escherichia coli in a micro‐cell was investigated by a dielectrophoretic impedance measurement (DEPIM) method. The temporal change in conductance across a micro‐gap (Δ G ) was measured for various heat treatment temperatures. In addition, the dependence of the enzyme activity, growth capacity, and membrane situation for E. coli on the heat treatment temperature was also analyzed by conventional biological methods. Consequently, a correlation between Δ G and those biological properties was obtained quantitatively. This result suggests that the DEPIM method will be an effective technique for monitoring complex changes in various biological states of microorganisms. © 2011 Wiley Periodicals, Inc. Electron Comm Jpn, 94(2): 9–16, 2011; Published online in Wiley Online Library ( wileyonlinelibrary.com ). DOI 10.1002/ecj.10321