Premium
An electrochemical sensor array and its application to real‐time brain slice imaging
Author(s) -
Kasai Nahoko,
Shimada Akiyoshi,
Nyberg Tobias,
Torimitsu Keiichi
Publication year - 2009
Publication title -
electronics and communications in japan
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.131
H-Index - 13
eISSN - 1942-9541
pISSN - 1942-9533
DOI - 10.1002/ecj.10140
Subject(s) - multielectrode array , horseradish peroxidase , electrode array , sensor array , chemistry , planar , electrode , planar array , materials science , nanotechnology , biomedical engineering , microelectrode , analytical chemistry (journal) , computer science , biochemistry , optics , physics , medicine , computer graphics (images) , machine learning , enzyme , chromatography
An electrochemical sensing system using a planar microelectrode array has been developed for monitoring biological molecules with relatively high spatial and temporal resolution. This allows noninvasive real‐time imaging of biological molecules released from tissue. In this study, we establish a multichannel hydrogen peroxide (H 2 O 2 ) sensing system to monitor the real‐time H 2 O 2 distribution in tissue using a planar sensor array. H 2 O 2 is known to be associated with the pathology of neurological diseases because it is a by‐product of degenerative reactions involving reactive oxygen species, which are a major cause of oxidative stress in mammalian cells. The sensor array is based on a 64‐channel ITO electrode array composed of 50 × 50 μm electrodes modified with an enzyme (horseradish peroxidase) and an electron transfer mediator. We place a cultured rat hippocampal slice on the array and measure the current at each sensor using a multipotentiostat. When we introduce bicuculline into the solution as a stimulant in the presence of a catalase inhibitor, we can observe a distinct increase in the H 2 O 2 concentration. This real‐time H 2 O 2 distribution monitoring system is a powerful tool with which to explore the neuronal cell death mechanism in biological systems. © 2009 Wiley Periodicals, Inc. Electron Comm Jpn, 92(9): 1–6, 2009; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/ecj.10140