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Validity of particle‐counting method using laser‐light scattering for detecting platelet aggregation in diabetic patients
Author(s) -
Nakadate Hiromichi,
Sekizuka Eiichi,
Minamitani Haruyuki
Publication year - 2009
Publication title -
electronics and communications in japan
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.131
H-Index - 13
eISSN - 1942-9541
pISSN - 1942-9533
DOI - 10.1002/ecj.10057
Subject(s) - platelet , platelet aggregation , scattering , agonist , light scattering , laser , particle (ecology) , platelet rich plasma , diabetes mellitus , chemistry , medicine , analytical chemistry (journal) , chromatography , optics , endocrinology , physics , receptor , oceanography , geology
We aimed to study the validity of a new analytical approach that reflected the phase from platelet activation to the formation of small platelet aggregates. We hoped that this new approach would enable us to use the particle‐counting method with laser‐light scattering to measure platelet aggregation in healthy controls and in diabetic patients without complications. We measured agonist‐induced platelet aggregation for 10 min. Agonist was added to the platelet‐rich plasma 1 min after measurement started. We compared the total scattered light intensity from small aggregates over a 10‐min period (established analytical approach) and at over a 2‐min period from 1 to 3 min after measurement started (new analytical approach). We found that platelet aggregation in diabetics with HbA lc ≥ 6.5% was significantly greater than in healthy controls by both analytical approaches. However, platelet aggregation in diabetics with HbA lc < 6.5%—patients in the early stages of diabetes—was significantly greater than in healthy controls only by the new analytical approach, not by the established analytical approach. These results suggest that platelet aggregation as detected by the particle‐counting method using laser‐light scattering could be applied in clinical examinations by our new analytical approach. © 2009 Wiley Periodicals, Inc. Electron Comm Jpn, 92(3): 1–8, 2009; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/ecj.10057