Open AccessA first‐generation microsatellite linkage map of the ruff
Author(s) -
Farrell Lindsay L.,
Burke Terry,
Slate Jon,
Lank David B.
Publication year - 2013
Publication title -
ecology and evolution
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.17
H-Index - 63
ISSN - 2045-7758
DOI - 10.1002/ece3.830
Subject(s) - zebra finch , microsatellite , biology , genetic linkage , genetics , genome , linkage (software) , taeniopygia , evolutionary biology , gene , allele , neuroscience
Abstract A linkage map of the ruff ( P hilomachus pugnax ) genome was constructed based on segregation analysis of 58 microsatellite loci from 381 captive‐bred individuals spanning fourteen breeding years and comprising 64 families. Twenty‐eight of the markers were resolved into seven linkage groups and five single marker loci, homologous to known chicken ( G allus gallus ) and zebra finch ( T aeniopygia guttata ) chromosomes. Linkage groups range from 10.1 to 488.7 cM in length and covered a total map distance of 641.6 cM, corresponding to an estimated 30–35% coverage of the ruff genome, with a mean spacing of 22.9 cM between loci. Through comparative mapping, we are able to assign linkage groups P pu1, P pu2, P pu6, P pu7, P pu10, P pu13, and P pu Z to chromosomes and identify several intrachromosomal rearrangements between the homologs of chicken, zebra finch, and ruff microsatellite loci. This is the first linkage map created in the ruff and is a major step toward providing genomic resources for this enigmatic species. It will provide an essential framework for mapping of phenotypically and behaviorally important loci in the ruff.