Open AccessIsolation and characterization of microsatellite markers for the threatened African endemic tree species Pterocarpus erinaceus Poir.
Author(s) -
Johnson Benziwa Nathalie,
Quashie Marie Luce Akossiwoa,
Chaix Gilles,
CamusKulandaivelu Letizia,
Adjonou Kossi,
Segla Kossi Novinyo,
Kokutse Adzo Dzifa,
Ouinsavi Christine,
Bationo Babou André,
Rabiou Habou,
Kokou Kouami,
Vignes Hélène
Publication year - 2020
Publication title -
ecology and evolution
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.17
H-Index - 63
ISSN - 2045-7758
DOI - 10.1002/ece3.6944
Subject(s) - microsatellite , biology , genetic diversity , threatened species , genetic structure , loss of heterozygosity , population , zoology , evolutionary biology , allele , genetic variation , ecology , genetics , gene , habitat , demography , sociology
To study the genetic diversity and structure of the forest species Pterocarpus erinaceus Poir., seventeen polymorphic nuclear microsatellite markers were isolated and characterized, using next‐generation sequencing. Three hundred and sixty‐five (365) individuals were analyzed within fifteen (15) West African populations. The number of alleles for these loci varied from 4 to 30, and the heterozygosity varied from 0.23 to 0.82. The seventeen (17) primers designed here will allow characterizing the genetic diversity of this threaten species on its natural stands and to better understand the population differentiation mechanisms shaping it.