Open AccessIsolation and characterization of fourteen polymorphic microsatellite markers in the viperine snake Natrix maura
Author(s) -
Le Chevalier Hugo,
MaríMeeus,
Carro Belén,
Prunier Jérôme G.,
Bossu Coralie,
Darnet Elodie,
Souchet Jérémie,
Guillaume Olivier,
Calvez Olivier,
Bertrand Romain,
Barthe Laurent,
Pottier Gilles,
MartínezSylvestre Albert,
VerdaguerFoz Isabel,
MossollTorres Marc,
Trochet Audrey,
Aubret Fabien
Publication year - 2019
Publication title -
ecology and evolution
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.17
H-Index - 63
ISSN - 2045-7758
DOI - 10.1002/ece3.5623
Subject(s) - microsatellite , linkage disequilibrium , biology , loss of heterozygosity , locus (genetics) , genetics , allele , natrix , genetic marker , evolutionary biology , disequilibrium , zoology , haplotype , gene , medicine , ophthalmology
Nineteen polymorphic microsatellite loci were identified and developed for Natrix maura . Polymorphism was assessed for 120 individuals sampled across four sampling sites from the French Pyrenees Mountains. The number of alleles per locus ranged from 3 to 15, and expected heterozygosity per locus ranged from 0.227 to 0.863. We tested for deviation from Hardy–Weinberg equilibrium and linkage disequilibrium and assessed the presence of null alleles for all loci, resulting in a selection of 14 high‐quality polymorphic markers. These markers will be extremely useful in identifying fine‐scale genetic structures and providing insight into conservation management plans of this species.