Open AccessGenome comparisons indicate recent transfer of w R i‐like Wolbachia between sister species Drosophila suzukii and D. subpulchrella
Author(s) -
Conner William R.,
Blaxter Mark L.,
Anfora Gianfranco,
Ometto Lino,
RotaStabelli Omar,
Turelli Michael
Publication year - 2017
Publication title -
ecology and evolution
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.17
H-Index - 63
ISSN - 2045-7758
DOI - 10.1002/ece3.3449
Subject(s) - wolbachia , biology , introgression , drosophila suzukii , drosophila (subgenus) , genome , genetics , sister group , evolutionary biology , gene , drosophila melanogaster , phylogenetics , drosophilidae , clade
Abstract Wolbachia endosymbionts may be acquired by horizontal transfer, by introgression through hybridization between closely related species, or by cladogenic retention during speciation. All three modes of acquisition have been demonstrated, but their relative frequency is largely unknown. Drosophila suzukii and its sister species D. subpulchrella harbor Wolbachia , denoted w S uz and w S pc, very closely related to w R i, identified in California populations of D. simulans . However, these variants differ in their induced phenotypes: w R i causes significant cytoplasmic incompatibility ( CI ) in D. simulans , but CI has not been detected in D. suzukii or D. subpulchrella . Our draft genomes of w S uz and w S pc contain full‐length copies of 703 of the 734 single‐copy genes found in w R i. Over these coding sequences, w S uz and w S pc differ by only 0.004% (i.e., 28 of 704,883 bp); they are sisters relative to w R i, from which each differs by 0.014%–0.015%. Using published data from D. melanogaster , Nasonia wasps and Nomada bees to calibrate relative rates of Wolbachia versus host nuclear divergence, we conclude that w S uz and w S pc are too similar—by at least a factor of 100—to be plausible candidates for cladogenic transmission. These three w R i‐like Wolbachia , which differ in CI phenotype in their native hosts, have different numbers of orthologs of genes postulated to contribute to CI ; and the CI loci differ at several nucleotides that may account for the CI difference. We discuss the general problem of distinguishing alternative modes of Wolbachia acquisition, focusing on the difficulties posed by limited knowledge of variation in absolute and relative rates of molecular evolution for host nuclear genomes, mitochondria, and Wolbachia .