Open AccessDetecting a hierarchical genetic population structure: the case study of the Fire Salamander ( Salamandra salamandra ) in Northern Italy
Author(s) -
Pisa Giulia,
Orioli Valerio,
Spilotros Giulia,
Fabbri Elena,
Randi Ettore,
Bani Luciano
Publication year - 2015
Publication title -
ecology and evolution
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.17
H-Index - 63
ISSN - 2045-7758
DOI - 10.1002/ece3.1335
Subject(s) - salamandra , genetic structure , biological dispersal , population , biology , ecology , salamander , genetic variation , zoology , demography , sociology
The multistep method here applied in studying the genetic structure of a low dispersal and philopatric species, such as the Fire Salamander Salamandra salamandra , was proved to be effective in identifying the hierarchical structure of populations living in broad‐leaved forest ecosystems in Northern Italy. In this study, 477 salamander larvae, collected in 28 sampling populations ( SP s) in the Prealpine and in the foothill areas of Northern Italy, were genotyped at 16 specie‐specific microsatellites. SP s showed a significant overall genetic variation (Global F ST = 0.032, P < 0.001). The genetic population structure was assessed by using STRUCTURE 2.3.4. We found two main genetic groups, one represented by SPs inhabiting the Prealpine belt, which maintain connections with those of the Eastern foothill lowland ( PEF ), and a second group with the SPs of the Western foothill lowland ( WF ). The two groups were significantly distinct with a Global F ST of 0.010 ( P < 0.001). While the first group showed a moderate structure, with only one divergent SP (Global F ST = 0.006, P < 0.001), the second group proved more structured being divided in four clusters (Global F ST = 0.017, P = 0.058). This genetic population structure should be due to the large conurbations and main roads that separate the WF group from the Prealpine belt and the Eastern foothill lowland. The adopted methods allowed the analysis of the genetic population structure of Fire Salamander from wide to local scale, identifying different degrees of genetic divergence of their populations derived from forest fragmentation induced by urban and infrastructure sprawl.