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Generation of Pecam1 endothelial specific dual reporter mouse model
Author(s) -
Hu Donghua,
Zhang Lu,
Yang Fan,
Chen Fuxue,
Li Deqiang,
Cai ChenLeng
Publication year - 2020
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.23384
Subject(s) - green fluorescent protein , microbiology and biotechnology , biology , endothelial stem cell , population , angiogenesis , endothelium , genetically modified mouse , transgene , in vitro , gene , cancer research , genetics , medicine , environmental health
Summary Endothelial cells are specialized epithelium lining the interior surface of vessels and play fundamental roles in angiogenesis, vascular permeability, and immune response. To identify endothelial cells in vivo, we constructed a Pecam1 nlacZ‐H2B‐GFP/+ knock‐in mouse model in which the endothelial cells are labeled by nuclear LacZ (nlacZ) expression. When Pecam1 nlacZ‐H2B‐GFP/+ mice are bred with germline Cre deleter mice, Pecam1 H2B‐GFP/+ line is created with native nuclear GFP (H2B‐GFP) expression in the endothelium of various organs. This dual reporter mouse provides us with a powerful genetic tool for definitive identification of endothelial cells and monitoring this important cell population throughout development, homeostasis, and disease conditions in mammals.