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Generation of an Fsp1 (fibroblast‐specific protein 1)‐Flpo transgenic mouse strain
Author(s) -
CardotRuffino Victoire,
Chauvet Véronique,
Caligaris Cassandre,
BertrandChapel Adrien,
Chuvin Nicolas,
Pommier Roxane M.,
Valcourt Ulrich,
Vincent David,
Martel Sylvie,
Aires Sophie,
Kaniewski Bastien,
Dubus Pierre,
Cassier Philippe,
Sentis Stéphanie,
Bartholin Laurent
Publication year - 2020
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.23359
Subject(s) - transgene , biology , recombinase , fibroblast , microbiology and biotechnology , phenotype , homologous recombination , site specific recombination , gastrulation , mesenchymal stem cell , gene , genetics , recombination , cell culture , embryonic stem cell
Summary Recombination systems represent a major breakthrough in the field of genetic model engineering. The Flp recombinases (Flp, Flpe, and Flpo) bind and cleave DNA Frt sites. We created a transgenic mouse strain ([Fsp1‐Flpo]) expressing the Flpo recombinase in fibroblasts. This strain was obtained by random insertion inside mouse zygotes after pronuclear injection. Flpo expression was placed under the control of the promoter of Fsp1 (fibroblast‐specific protein 1) gene, whose expression starts after gastrulation at Day 8.5 in cells of mesenchymal origin. We verified the correct expression and function of the Flpo enzyme by several ex vivo and in vivo approaches. The [Fsp1‐Flpo] strain represents a genuine tool to further target the recombination of transgenes with Frt sites specifically in cells of mesenchymal origin or with a fibroblastic phenotype.