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Generation and characterization of Megf6 null and Cre knock‐in alleles
Author(s) -
Wang Ye,
Song Hejie,
Wang Wenfeng,
Zhang Zhen
Publication year - 2019
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.23262
Subject(s) - biology , mesenchyme , allele , null allele , embryonic stem cell , crispr , gene knockin , genetics , microbiology and biotechnology , conditional gene knockout , homologous chromosome , phenotype , gene , embryo
Summary Megf6, a member of MEGF (multiple EGF‐like domains) protein family, is a conserved high molecular weight protein with 30 EGF‐like domains. Although many members of the MEGF protein family are essential for embryonic development and homeostasis, the role of Megf6 in development and physiology is still unknown. Here, we generated Megf6‐deficient mice using CRISPR‐Cas9 technique and showed that Megf6 is dispensable for embryonic development. We also constructed the Megf6 Cre allele to study Megf6‐expressing cell lineages. Our results showed that Megf6‐expressing cells contribute to the periotic mesenchyme and its derivatives, skin epidermis, certain cells in brain and ribs. Therefore, the Megf6 Cre allele can be a useful tool for conditional deletion in these tissues, in particular for periotic mesenchyme deletion.