Premium
Generation of MLC‐2v‐tdTomato knock‐in reporter mouse line
Author(s) -
Zhang Zhentao,
Nam YoungJae
Publication year - 2018
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.23256
Subject(s) - myogenesis , biology , myocyte , transgene , skeletal muscle , microbiology and biotechnology , gene knockin , genetically modified mouse , heart development , myofibril , myosin , reporter gene , embryonic stem cell , endogeny , green fluorescent protein , anatomy , gene expression , gene , endocrinology , genetics
Summary MLC‐2v is a myosin light chain regulatory protein which is specifically expressed in ventricular cardiomyocytes and slow twitch skeletal muscle cells. MLC‐2v plays critical roles in ventricular maturation during heart development. Mice lacking MLC‐2v are embryonic lethal due to heart failure associated with abnormal myofibrillar organization of ventricular cardiomyocytes. To study the development of ventricular cardiac muscle and slow twitch skeletal muscle, we generated a new MLC‐2v reporter mouse line by knocking‐in a tdTomato reporter cassette into 3′ UTR of the MLC‐2v gene without disrupting the endogenous gene. Our results demonstrated specific MLC‐2v ‐ tdTomato knock‐in reporter expression in ventricular cardiomyocytes and slow twitch muscle during myogenesis, precisely recapitulating the spatiotemporal expression pattern of endogenous MLC‐2v. No tdTomato expression was observed in the atria, fast twitch muscle or other organs throughout development into adulthood. Isolated neonatal and adult ventricular cardiomyocytes uniformly express tdTomato. Taken together, MLC‐2v‐tdTomato knock‐in reporter mouse model described in this article will serve as a valuable tool to study cardiac chamber and skeletal muscle specification during development and regeneration by overcoming the pitfalls of transgenic strategies.