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A novel mouse Cre‐driver line targeting Perilipin 2‐expressing cells in the neonatal lung
Author(s) -
Ntokou Aglaia,
Szibor Marten,
RodríguezCastillo José Alberto,
Quantius Jennifer,
Herold Susanne,
El Agha Elie,
Bellusci Saverio,
Salwig Isabelle,
Braun Thomas,
Voswinckel Robert,
Seeger Werner,
Morty Rory E.,
Ahlbrecht Katrin
Publication year - 2017
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.23080
Subject(s) - lung , biology , mesenchymal stem cell , microbiology and biotechnology , pulmonary fibrosis , fibrosis , pathology , cancer research , medicine
Summary Pulmonary diseases such as chronic obstructive pulmonary disease, lung fibrosis, and bronchopulmonary dysplasia are characterized by the destruction or malformation of the alveolar regions of the lung. The underlying pathomechanisms at play are an area of intense interest since these mechanisms may reveal pathways suitable for interventions to drive reparative processes. Lipid‐laden fibroblasts (lipofibroblasts) express the Perilipin 2 ( Plin2 ) gene‐product, PLIN2, commonly called adipose‐differentiation related protein (ADRP). These cells are also thought to play a role in alveolarization and repair after injury to the alveolus. Progress in defining the functional contribution of lipofibroblasts to alveolar generation and repair is hampered by a lack of in vivo tools. The present study reports the generation of an inducible mouse Cre‐driver line to target cells of the ADRP lineage. Robust Cre‐mediated recombination in this mouse line was detected in mesenchymal cells of the postnatal lung, and in additional organs including the heart, liver, and spleen. The generation and validation of this valuable new tool to genetically target, manipulate, and trace cells of the ADRP lineage is critical for assessing the functional contribution of lipofibroblasts to lung development and repair.