Premium
The Drosophila melanogaster Eip74EF‐PA transcription factor directly binds the sciarid BhC4‐1 promoter
Author(s) -
Frank Henrique Oliveira,
Sanchez Danilo Garcia,
Freitas Oliveira Lucas,
Kobarg Jörg,
Monesi Nadia
Publication year - 2017
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.23075
Subject(s) - drosophila melanogaster , biology , ecdysone , melanogaster , transcription factor , gene , gene isoform , metamorphosis , transgene , transcription (linguistics) , imaginal disc , gene expression , promoter , microbiology and biotechnology , genetics , regulation of gene expression , larva , linguistics , philosophy , botany
Summary The DNA puff BhC4‐1 gene of Bradysia hygida (Diptera, Sciaridae) is amplified and expressed in the salivary glands at the end of the last larval instar. Even though there are no BhC4‐1 orthologs in Drosophila melanogaster , the mechanisms that regulate BhC4‐1 gene expression in B. hygida are for the most part conserved in D. melanogaster . The BhC4‐1 promoter contains a 129bp (‐186/‐58) cis ‐regulatory module (CRM) that drives developmentally regulated expression in transgenic salivary glands at the onset of metamorphosis. Both in the sciarid and in transgenic D. melanogaster , BhC4‐1 gene expression is induced by the increase in ecdysone titers that triggers metamorphosis. Genetic interaction experiments revealed that in the absence of the Eip74EF‐PA early gene isoform BhC4‐1‐lacZ levels of expression in the salivary gland are severely reduced. Here we show that the overexpression of the Eip74EF‐PA transcription factor is sufficient to anticipate BhC4‐1‐lacZ expression in transgenic D. melanogaster . Through yeast one‐hybrid assays we confirm that the Eip74EF‐PA transcription factor directly binds to the 129 bp sciarid CRM. Together, these results contribute to the characterization of an insect CRM and indicate that the ecdysone gene regulatory network that promotes metamorphosis is conserved between D. melanogaster and the sciarid B. hygida .