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Chip/Ldb1 interacts with Tailup/islet1 to regulate cardiac gene expression in Drosophila
Author(s) -
Werner Kathrin,
Donow Cornelia,
Pandur Petra
Publication year - 2017
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.23030
Subject(s) - transcription factor , biology , enhancer , heart development , microbiology and biotechnology , context (archaeology) , cell fate determination , regulation of gene expression , gene expression , gene , homeobox , genetics , embryonic stem cell , paleontology
The LIM‐homeodomain transcription factor Tailup (Tup) is a component of the complex cardiac transcriptional network governing specification and differentiation of cardiac cells in Drosophila . LIM‐domain containing factors are known to interact with the adaptor molecule Chip/Ldb1 to form higher order protein complexes to regulate gene expression thereby determining a cell's developmental fate. However, with respect to Drosophila heart development, it has not been investigated yet, whether Chip and tup interact to regulate the generation of different cardiac cell types. Here we show that Chip is required for normal heart development and that it interacts with tup in this context. Particularly the number of Odd skipped‐expressing pericardial cells depends on balanced amounts of Chip and Tup. Data from luciferase assays using Hand ‐ and even‐skipped reporter constructs in Drosophila S2 cells indicate that Chip and Tup act as a tetrameric complex on the regulatory regions of Hand and even‐skipped ( eve ). Finally we have identified and verified five Tup binding sites in the eve mesodermal enhancer, which adds Tup as novel factor to directly regulate eve expression. Taken together this study provides novel findings regarding cardiac gene expression regulation in Drosophila .