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Targeted integration of genes in Xenopus tropicalis
Author(s) -
Shi Zhaoying,
Tian Dandan,
Xin Huhu,
Lian Jingru,
Guo Xiaogang,
Chen Yonglong
Publication year - 2017
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.23006
Subject(s) - xenopus , biology , computational biology , gene , homologous recombination , genetics , homology (biology) , homologous chromosome , gene targeting
With the successful establishment of both targeted gene disruption and integration methods in the true diploid frog Xenopus tropicalis , this excellent vertebrate genetic model now is making a unique contribution to modelling human diseases. Here, we summarize our efforts on establishing homologous recombination‐mediated targeted integration in Xenopus tropicalis , the usefulness, and limitation of targeted integration via the homology‐independent strategy, and future directions on how to further improve targeted gene integration in Xenopus tropicalis .