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Germline recombination in a novel Cre transgenic line, Prl3b1 ‐Cre mouse
Author(s) -
AlSoudy AlSayed,
Nakanishi Tsuyoshi,
Mizuno Seiya,
Hasegawa Yoshikazu,
Shawki Hossam H.,
Katoh Megumi C.,
Basha Walaa A.,
Ibrahim Abdelaziz E.,
ElShemy Hany A.,
Iseki Hiroyoshi,
Yoshiki Atsushi,
Hiromori Youhei,
Nagase Hisamitsu,
Takahashi Satoru,
Oishi Hisashi,
Sugiyama Fumihiro
Publication year - 2016
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.22944
Subject(s) - cre recombinase , biology , transgene , germline , genetically modified mouse , spermatogenesis , microbiology and biotechnology , gene targeting , germ cell , green fluorescent protein , gene , genetics , endocrinology
Summary Spermatogenesis is a complex and highly regulated process by which spermatogonial stem cells differentiate into spermatozoa. To better understand the molecular mechanisms of the process, the Cre/loxP system has been widely utilized for conditional gene knockout in mice. In this study, we generated a transgenic mouse line that expresses Cre recombinase under the control of the 2.5 kbp of the Prolactin family 3, subfamily b, member 1 ( Prl3b1 ) gene promoter ( Prl3b1 ‐cre). Prl3b1 was initially reported to code for placental lactogen 2 (PL‐2) protein in placenta along with increased expression toward the end of pregnancy. PL‐2 was found to be expressed in germ cells in the testis, especially in spermatocytes. To analyze the specificity and efficiency of Cre recombinase activity in Prl3b1 ‐cre mice, the mice were mated with reporter R26GRR mice, which express GFP ubiquitously before and tdsRed exclusively after Cre recombination. The systemic examination of Prl3b1 ‐cre;R26GRR mice revealed that tdsRed‐positive cells were detected only in the testis and epididymis. Fluorescence imaging of Prl3b1 ‐cre;R26GRR testes suggested that Cre‐mediated recombination took place in the germ cells with approximately 74% efficiency determined by in vitro fertilization. In conclusion, our results suggest that the Prl3b1 ‐ cre mice line provides a unique resource to understand testicular germ‐cell development. genesis 54:389–397, 2016. © 2016 Wiley Periodicals, Inc.