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Cell type‐specific epigenome profiling using affinity‐purified nuclei
Author(s) -
Chitikova Zhanna,
Steiner Florian A.
Publication year - 2016
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.22919
Subject(s) - chromatin , biology , multicellular organism , epigenome , cell type , cellular differentiation , progenitor cell , computational biology , gene expression profiling , rna , microbiology and biotechnology , gene expression , zygote , cell , gene , genetics , stem cell , dna methylation , embryogenesis
Summary The development of a multicellular organism from a single zygote depends on the differentiation of progenitor cells to specialized cell types. The differentiation of these cell types is associated with changes in gene expression and the underlying chromatin landscape. To understand how these processes are regulated, it is desirable to understand how the chromatin features that constitute the epigenome differ between cell types at any given time during development. INTACT, a method for the cell type‐specific purification of nuclei that can be used for the isolation of both RNA and chromatin, has emerged as a powerful tool to simultaneously study gene expression and chromatin profiles specifically in cell types of interest. In this review, we focus on the application of INTACT to different model organisms and discuss its potential for profiling cell types in their developmental context. genesis, 2016. © 2016 Wiley Periodicals, Inc. genesis 54:160–169, 2016. © 2016 Wiley Periodicals, Inc.