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Grhl2 is required in nonneural tissues for neural progenitor survival and forebrain development
Author(s) -
Menke Chelsea,
Cionni Megan,
Siggers Trevor,
Bulyk Martha L.,
Beier David R.,
Stottmann Rolf W.
Publication year - 2015
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.22875
Subject(s) - biology , ectoderm , forebrain , progenitor cell , organogenesis , progenitor , phenotype , genetics , neural development , mutant , microbiology and biotechnology , neural stem cell , allele , gene , embryonic stem cell , stem cell , neuroscience , central nervous system
Summary Grainyhead‐like genes are part of a highly conserved gene family that play a number of roles in ectoderm development and maintenance in mammals. Here we identify a novel allele of Grhl2 , cleft‐face 3 ( clft3 ), in a mouse line recovered from an ENU mutagenesis screen for organogenesis defects. Homozygous clft3 mutants have a number of phenotypes in common with other alleles of Grhl2 . We note a significant effect of genetic background on the clft3 phenotype. One of these is a reduction in size of the telencephalon where we find abnormal patterns of neural progenitor mitosis and apoptosis in mutant brains. Interestingly, Grhl2 is not expressed in the developing forebrain, suggesting this is a survival factor for neural progenitors exerting a paracrine effect on the neural tissue from the overlying ectoderm where Grhl2 is highly expressed. genesis 53:573–582, 2015. © 2015 Wiley Periodicals, Inc.