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Derivation of chondrocyte and osteoblast reporter mouse embryonic stem cell lines
Author(s) -
Fu Yu,
Maye Peter
Publication year - 2015
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.22849
Subject(s) - embryonic stem cell , microbiology and biotechnology , chondrocyte , osteoblast , stem cell , biology , anatomy , genetics , cartilage , gene , in vitro
Summary With the establishment of methods that provide evidence for the generation of chondrocyte and osteoblast cell types from ESCs, there is a need for reagents that will enable their further characterization. Here we report on the derivation of chondrocyte and osteoblast reporter ESCs from previously generated and characterized transgenic mouse lines, Collagen type 2 alpha 1(Col2a1)‐ECFP , Bone Sialoprotein (BSP)‐Topaz , and BSP‐Topaz/Dentin Matrix Protein 1 (DMP1)‐Cherry dual reporter mice. Col2a1 ‐ECFP is highly expressed in chondrocytes, while BSP ‐Topaz and DMP1 ‐Cherry are highly expressed in osteoblasts and osteocytes, respectively. These new skeletal reporter mouse ESC lines will serve as valuable reagents to investigate the functionality of ESC derived chondrocyte and osteoblast cell types. genesis 53:294–298, 2015. © 2015 Wiley Periodicals, Inc.