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Generation and characterization of KsprtTA and KsptTA transgenic mice
Author(s) -
Pan Xinchao,
Small Erin V.,
Igarashi Peter,
Carroll Thomas J.
Publication year - 2013
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.22381
Subject(s) - doxycycline , transgene , genetically modified mouse , biology , gene , microbiology and biotechnology , transcription (linguistics) , embryonic stem cell , gene expression , kidney , transcription factor , genetics , antibiotics , linguistics , philosophy
The advent of technologies that allow tissue specific expression or ablation of genes has contributed enormously to our knowledge of the mechanism regulating organ development and maintenance in mice. The tetracycline inducible system allows reversible regulation of gene products upon administration of doxycycline. Here we describe the generation and activity of two transgenic lines expressing the cDNAs for the Tet responsive transcription factors rtTA and tTA (Tet‐on and off) respectively under the control of an element that drives expression in the epithelium of the developing and adult kidney. Both lines show inducible and reversible activity in the embryonic and adult organ. genesis 51:430–435. © 2013 Wiley Periodicals, Inc.