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Pax1 EGFP : New wildtype and mutant EGFP mouse lines for molecular and fate mapping studies
Author(s) -
Sivakamasundari V.,
Kraus Petra,
Jie Song,
Lufkin Thomas
Publication year - 2013
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.22379
Subject(s) - biology , green fluorescent protein , mutant , wild type , microbiology and biotechnology , gene , genetics
Summary The Paired box gene 1 ( Pax1 ) transcription factor plays essential roles in the development of axial skeleton, scapula, pelvic girdle, and thymus. Delineating its pleiotropic and molecular roles in the various tissues requires the ability to track and isolate the Pax1 ‐expressing cells for downstream high‐throughput experiments such as microarray and RNA‐sequencing. With these applications in mind, we have generated two new mouse lines—a Pax1 wildtype (WT) mouse line that co‐expresses enhanced green fluorescent protein (EGFP) with functional Pax1, and a Pax1 knockout mouse line which expresses EGFP under the control of Pax1 promoter, using the internal ribosome entry site (IRES) and 2A‐peptide multi‐cistron concatenating strategies. These mouse lines facilitate the isolation and enrichment of Pax1 ‐specific cells from Pax1 ‐positive and Pax1 ‐null embryos using fluorescence activated cell sorting (FACS). They can be also be used in parallel to investigate the stage‐ and tissue‐specific molecular functions of Pax1 . genesis 51:420–429. © 2013 Wiley Periodicals, Inc.