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Experimental approaches for gene regulatory network construction: The chick as a model system
Author(s) -
Streit Andrea,
Tambalo Monica,
Chen Jingchen,
Grocott Timothy,
Anwar Maryam,
Sosinsky Alona,
Stern Claudio D.
Publication year - 2013
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.22359
Subject(s) - construct (python library) , body plan , gene regulatory network , workflow , key (lock) , computer science , computational biology , biology , gene , control (management) , artificial intelligence , genetics , gene expression , computer network , computer security , database
Setting up the body plan during embryonic development requires the coordinated action of many signals and transcriptional regulators in a precise temporal sequence and spatial pattern. The last decades have seen an explosion of information describing the molecular control of many developmental processes. The next challenge is to integrate this information into logic “wiring diagrams” that visualize gene actions and outputs, have predictive power and point to key control nodes. Here, we provide an experimental workflow on how to construct gene regulatory networks using the chick as model system. genesis 51:296–310, 2012. © 2012 Wiley Periodicals, Inc.