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Production of a mouse line with a conditional Crim1 mutant allele
Author(s) -
Chiu Han Sheng,
York J. Philippe,
Wilkinson Lorine,
Zhang Pumin,
Little Melissa H.,
Pennisi David J.
Publication year - 2012
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.22032
Subject(s) - mutant , allele , genetics , biology , line (geometry) , gene , mathematics , geometry
Crim1 is a developmentally expressed, transmembrane protein essential for normal embryonic development. We generated mice engineered to contain a Crim1 conditional null allele by flanking exons three and four of Crim1 with unidirectional LoxP sites. After crossing Crim1 +/FLOX mice with a CMV‐Cre line, a Crim1 +/Δflox colony was established after germline transmission of the deleted allele. We then analyzed genomic DNA, mRNA transcripts, and protein expression from Crim1 Δflox/Δflox null mice to confirm the nature of the genomic lesion. Crim1 Δflox/Δflox mice displayed phenotypes similar to those previously described for a Crim1 gene‐trap mutant, Crim1 KST264/KST264 , including perinatal lethality, digit syndactyly, eye, and kidney abnormalities, with varying penetrance and severity. The production of a conditional mutant allele represents a valuable resource for the study of the tissue‐specific roles for Crim1 , and for understanding the pleimorphic phenotypes associated with Crim1 mutation. genesis 50:711–716, 2012. © 2012 Wiley Periodicals, Inc.