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In vivo visualization of the lymphatic vessels in pFLT4‐EGFP transgenic medaka
Author(s) -
Deguchi Tomonori,
Fujimori Kazuhiro E.,
Kawasaki Takashi,
Maruyama Kouichi,
Yuba Shunsuke
Publication year - 2012
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.22018
Subject(s) - biology , green fluorescent protein , lymphatic system , in situ hybridization , transgene , microbiology and biotechnology , genetically modified mouse , oryzias , high endothelial venules , lymphatic endothelium , in vivo , fluorescence in situ hybridization , pathology , gene expression , gene , genetics , immunology , medicine , chromosome
Feline McDonough Sarcoma (FMS)‐like tyrosine kinase 4 (FLT4) is a marker for lymphatic vessels and some high endothelial venules in human adult tissues. We generated a transgenic medaka fish in which the lymphatic vessels and some blood vessels are visible in vivo by transferring the promoter of medaka flt4 driving the expression of enhanced green fluorescent protein (EGFP) using a see‐through medaka line. To do this, we identified and cloned medaka flt4 and generated a construct in which the promoter was the 4‐kb region upstream of the translation initiation site. The fluorescent signal of EGFP could be observed with little background, and the expression pattern correlated well with that of flt4 determined by whole‐mount RNA in situ hybridization. Because a see‐through medaka line is transparent until adult, the model is useful for visualizing the lymphatic vessels not only in embryo and fry but also in adult. This model will be a useful tool for analyzing lymphatic development. genesis 50:625–634, 2012. © 2012 Wiley Periodicals, Inc.