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Drosophila DREF acting via the JNK pathway is required for thorax development
Author(s) -
Yoshioka Yasuhide,
Tue Nguyen Trong,
Fujiwara Shunsuke,
Matsuda Risa,
ValadezGraham Viviana,
Zurita Mario,
Yamaguchi Masamitsu
Publication year - 2012
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.22017
Subject(s) - gene knockdown , biology , chromatin immunoprecipitation , promoter , enhancer , gene , microbiology and biotechnology , luciferase , schneider 2 cells , gene expression , rna interference , genetics , rna , transfection
The Drosophila Jun N‐terminal kinase ( JNK ) gene basket ( bsk ) promoter contains a DNA replication‐related element (DRE)‐like sequence, raising the possibility of regulation by the DNA replication‐related element‐binding factor (DREF). Chromatin immunoprecipitation assays with anti‐DREF IgG showed the bsk gene promoter region to be effectively amplified. Luciferase transient expression assays revealed the DRE‐like sequence to be important for bsk gene promoter activity, and knockdown of DREF decreased the bsk mRNA level and the bsk gene promoter activity. Furthermore, knockdown of DREF in the notum compartment of wing discs by pannier ‐GAL4 and UAS‐ DREFIR resulted in a split thorax phenotype. Monitoring of JNK activity in the wing disc by LacZ expression in a puckered (puc)‐LacZ enhancer trap line revealed the reduction in DREF knockdown clones. These findings indicate that DREF is involved in regulation of Drosophila thorax development via actions on the JNK pathway. genesis 50:599–611, 2012. © 2012 Wiley Periodicals, Inc.