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Characterization of small RNAs in Xenopus tropicalis gastrulae
Author(s) -
Faunes Fernando,
Almonacid Leonardo I.,
Melo Francisco,
Larrain Juan
Publication year - 2012
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.22012
Subject(s) - biology , xenopus , piwi interacting rna , transposable element , small rna , genome , microrna , argonaute , gastrulation , computational biology , rna , genetics , embryo , embryogenesis , gene , rna interference
Here, we report and characterize deep sequencing data and bioinformatics analysis of small RNAs from Xenopus tropicalis gastrula. A total of 17,553,124 reads with perfect match to the genome derived from 2,616,053 unique sequences were identified. Seventy‐seven percent of theses sequences were not found in previous reports from X. tropicalis oocytes and somatic tissues. Bioinformatics analyses indicate that a large fraction of the small RNAs are PIWI‐interacting RNAs. Up to 23.9% of small RNAs mapped to transposable elements and 27% to genic regions. Most of abundant transposable derived small RNAs are found in oocyte and gastrula libraries, suggesting that transposon needs to be silenced also during early development. Additionally, miRNAs were identified and many of them are not present in oocytes, suggesting that miRNA expression is stage specific. To the best of our knowledge, this is the first high throughput data release and bioinformatics characterization of small RNAs during Xenopus development. genesis 50:260–270, 2012. © 2012 Wiley Periodicals, Inc.