Premium
From expression cloning to gene modeling: The development of Xenopus gene sequence resources
Author(s) -
Gilchrist Michael J.
Publication year - 2012
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.22008
Subject(s) - xenopus , biology , gene , cloning (programming) , complementary dna , genetics , genome , computational biology , coding region , clone (java method) , sequence (biology) , model organism , computer science , programming language
The Xenopus community has made concerted efforts over the last 10–12 years systematically to improve the available sequence information for this amphibian model organism ideally suited to the study of early development in vertebrates. Here I review progress in the collection of both sequence data and physical clone reagents for protein coding genes. I conclude that we have cDNA sequences for around 50% and full‐length clones for about 35% of the genes in Xenopus tropicalis , and similar numbers but a smaller proportion for Xenopus laevis . In addition, I demonstrate that the gaps in the current genome assembly create problems for the computational elucidation of gene sequences, and suggest some ways to ameliorate the effects of this. genesis 50:143–154, 2012. © 2012 Wiley Periodicals, Inc.