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Site‐specific transgenesis in Xenopus
Author(s) -
Zuber Michael E.,
Nihart Heather S.,
Zhuo Xinming,
Babu Sudha,
Knox Barry E.
Publication year - 2012
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.22006
Subject(s) - xenopus , transgenesis , biology , transgene , enhancer , site specific recombination , promoter , genetics , cre recombinase , plasmid , gene , microbiology and biotechnology , computational biology , recombinase , transcription factor , gene expression , genetically modified mouse , recombination , reproductive technology , embryogenesis
Abstract Transgenesis is an essential, powerful tool for investigating gene function and the activities of enhancers, promoters, and transcription factors in the chromatin environment. In Xenopus , current methods generate germ‐line transgenics by random insertion, often resulting in mosaicism, position‐dependent variations in expression, and lab‐to‐lab differences in efficiency. We have developed and tested a Xenopus FLP‐FRT recombinase‐mediated transgenesis (X‐FRMT) method. We demonstrate transgenesis of Xenopus laevis by FLP‐catalyzed recombination of donor plasmid cassettes into F 1 tadpoles with host cassette transgenes. X‐FRMT provides a new method for generating transgenic Xenopus . Once Xenopus lines harboring single host cassettes are generated, X ‐FRMT should allow for the targeting of transgenes to well‐characterized integration site(s), requiring no more special reagents or training than that already common to most Xenopus labs. genesis 50:325–332, 2012. © 2012 Wiley Periodicals, Inc.